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Dog10K_Boxer_Tasha_1.0: A Long-Read Assembly of the Dog Reference Genome

Abstract : The domestic dog has evolved to be an important biomedical model for studies regarding the genetic basis of disease, morphology and behavior. Genetic studies in the dog have relied on a draft reference genome of a purebred female boxer dog named "Tasha" initially published in 2005. Derived from a Sanger whole genome shotgun sequencing approach coupled with limited clone-based sequencing, the initial assembly and subsequent updates have served as the predominant resource for canine genetics for 15 years. While the initial assembly produced a good-quality draft, as with all assemblies produced at the time, it contained gaps, assembly errors and missing sequences, particularly in GC-rich regions, which are found at many promoters and in the first exons of protein-coding genes. Here, we present Dog10K_Boxer_Tasha_1.0, an improved chromosome-level highly contiguous genome assembly of Tasha created with long-read technologies that increases sequence contiguity >100-fold, closes >23,000 gaps of the CanFam3.1 reference assembly and improves gene annotation by identifying >1200 new protein-coding transcripts. The assembly and annotation are available at NCBI under the accession GCF_000002285.5.
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https://hal.archives-ouvertes.fr/hal-03260719
Contributor : Xavier Chard-Hutchinson <>
Submitted on : Tuesday, June 15, 2021 - 10:30:46 AM
Last modification on : Friday, June 18, 2021 - 3:43:27 AM

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Vidhya Jagannathan, Christophe Hitte, Jeffrey M Kidd, Patrick Masterson, Terence D Murphy, et al.. Dog10K_Boxer_Tasha_1.0: A Long-Read Assembly of the Dog Reference Genome. Genes, MDPI, 2021, 12 (6), pp.847. ⟨10.3390/genes12060847⟩. ⟨hal-03260719⟩

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