Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology. - Centre de recherche en cancérologie Nantes-Angers Unité Mixte de Recherche 892 Inserm - 6299 CNRS Accéder directement au contenu
Article Dans Une Revue Glycobiology Année : 2011

Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology.

Résumé

The growth and differentiation of bone cells is controlled by various factors, which can be modulated by heparan sulfates. Here, we investigated the effects of an oversulfated exopolysaccharide (OS-EPS) on the bone. We compared the effect of this compound with that of a native EPS. Long-term administration of OS-EPS causes cancellous bone loss in mice due, in part, to an increase in the number of osteoclasts lining the trabecular bone surface. No significant difference in cancellous bone volume was found between EPS-treated mice and age-matched control mice, underlying the importance of sulfation in trabecular bone loss. However, the mechanism sustaining this osteoporosis was unclear. To clarify OS-EPS activities, we investigated the effect of OS-EPS on osteogenesis. Our results demonstrated that OS-EPS inhibited osteoclastogenesis in two cell models. Using the surface plasmon resonance technique, we revealed that OS-EPS can form a hetero-molecular complex OS-EPS/receptor activator of NF-κB ligand (RANKL)/RANK and that RANK had a higher affinity for RANKL pre-incubated with OS-EPS than for RANKL alone, which would be in favor of an increase in bone resorption. However, in vitro, OS-EPS inhibited the early steps of osteoclast precursor adhesion and therefore inhibited the cell fusion step. In addition, we showed that OS-EPS reduced proliferation and accelerated osteoblastic differentiation, leading to strong inhibition of mineralized nodule formation, which would be in favor of an increase in bone resorption. Taken together, these data show different levels of bone resorption regulation by EPSs, most of them leading to proresorptive effects.
Fichier principal
Vignette du fichier
Ruiz_Velasco_et_al.pdf (1.73 Mo) Télécharger le fichier
inserm-00667495_edited.pdf (703.91 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

inserm-00667495 , version 1 (12-03-2012)

Identifiants

Citer

Carmen Ruiz-Velasco, Marc Baud'Huin, Corinne Sinquin, Mike Maillasson, Dominique Heymann, et al.. Effects of a sulfated exopolysaccharide produced by Altermonas infernus on bone biology.. Glycobiology, 2011, 21 (6), pp.781-95. ⟨10.1093/glycob/cwr002⟩. ⟨inserm-00667495⟩
182 Consultations
753 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More