Circulating Cd34+ cell count differentiates primary myelofibrosis from other Philadelphia-negative myeloproliferative neoplasms: a pragmatic study

Corentin Orvain (1, 2, 3) , Damien Luque Paz (4, 5, 2) , Irène Dobo (2) , Laurane Cottin (2) , Geneviève Le Calvez (5) , Aurélie Chauveau (5) , Mélanie Mercier (3) , J. Farhi (3) , Françoise Boyer (3) , Jean-Christophe Ianotto (6) , Briac Guibourg (7) , Marie- Christine Rousselet (4) , Marc Zandecki (2) , Norbert Ifrah (1) , Mathilde Hunault-Berger (1, 3) , Valérie Ugo (1, 5, 4, 2) , Franck Genevieve (2)

1 CRCNA - Centre de Recherche en Cancérologie Nantes-Angers
2 Laboratoire d'hématologie
3 Service des maladies du sang
4 UA - Université d'Angers
5 Labo Hémato - BREST - Laboratoire d'hématologie
6 Institut de cancérologie et d'hématologie
7 CHRU Brest - Centre Hospitalier Régional Universitaire de Brest

A high number of circulating CD34+ cells has been advocated to distinguish primary myelofibrosis from other Philadelphia-negative myeloproliferative neoplasms. We reevaluated the diagnostic interest of measuring circulating CD34+ cells in 26 healthy volunteers and 256 consecutive patients at diagnosis for whom a myeloproliferative neoplasm was suspected. The ROC curve analysis showed that a number of CD34+ <10/μl excludes the diagnosis of primary myelofibrosis with a sensitivity of 97%and a specificity of 90%(area under the curve: 0.93 [0.89–0.98]; p < 0.001). Patients with PMF harboring a CALR mutation had more circulating CD34+ cells than patients with either a JAK 2 or MPL mutation (p = 0.02 and p < 0.01, respectively). These results suggest that this fast, simple, non-invasive, and standardized test is of particular interest to exclude the diagnosis of primary myelofibrosis.

Domaines: Cancer

Corentin Orvain, Damien Luque Paz, Irène Dobo, Laurane Cottin, Geneviève Le Calvez, et al.. Circulating Cd34+ cell count differentiates primary myelofibrosis from other Philadelphia-negative myeloproliferative neoplasms: a pragmatic study. Annals of Hematology, 2016, 95 ((11)), pp.1819-23. ⟨10.1007/s00277-016-2784-x⟩. ⟨inserm-01382431⟩