Assessing patterns for compressive fluorescence lifetime imaging

M. Ochoa (1) , Q. Pian (1) , R. Yao (1) , Nicolas Ducros (2) , X. Intes (1)

1 RPI - Rensselaer Polytechnic Institute
2 Imagerie Tomographique et Radiothérapie

A novel hyperspectral single pixel system was used to compare different compressive basis patterns for intensity imaging, lifetime imaging, and FRET quantification. Six popular basis patterns were compared experimentally in a phantom containing two fluorescent dyes. The basis patterns that performed best for lifetime quantification were used to measure FRET occurrence in well-plate samples with varying acceptor-donor ratios. The ABS-WP approach using Haar patterns and the compressive sensing approach with Hadamard Ranked patterns displayed the best overall performances at a 50% compression ratio.

Domaines: Imagerie médicale; Traitement du signal et de l'image [eess.SP]; Optique / photonique

M. Ochoa, Q. Pian, R. Yao, Nicolas Ducros, X. Intes. Assessing patterns for compressive fluorescence lifetime imaging. Optics Letters, 2018, 43 (18), pp.4370-4373. ⟨10.1364/OL.43.004370⟩. ⟨hal-01922724⟩