Determination of 9-hydroxyellipticine by redox colorimetry

Pascal Formisyn (1, 2) , Félix Pautet (3) , Canh Tran Minh (2, 1, 4, 5) , Jacques Bourgois (2, 1)

1 LB-ENSMSE - Laboratoire de Biotechnologie
2 Mines Saint-Étienne MSE - École des Mines de Saint-Étienne
3 LCO - Laboratoire de Chimie Organique
4 SPIN-ENSMSE - Centre Sciences des Processus Industriels et Naturels
5 P2MC-ENSMSE - Département Poudres et Matériaux Multi-Composants

The chemical reactions involved in the decomposition of 9-hydroxyellipticine (9-OH-E), an anticancer agent, in polar solvents is explained. The reactions, which involve the formation of 9-oxo-ellipticine and the addition of a nucleophilic acid on the C10 site of the heterocyclic system, have been used to measure 9-OH-E quantitatively by colorimetry in solution and by reflection on paper surfaces. A method for the stabilization of 9-OH-E in polar solvents is proposed.

Mots clés: 9-Hydroxyellipticine; mechanism of instability; qualitative and quantitative analysis; thin-layer chromatography; colour measurement; stabilization

Domaines: Génie des procédés; Biotechnologies; Biotechnologie

Pascal Formisyn, Félix Pautet, Canh Tran Minh, Jacques Bourgois. Determination of 9-hydroxyellipticine by redox colorimetry. Journal of Pharmaceutical and Biomedical Analysis, 1992, 10 (6), pp.427-436. ⟨10.1016/0731-7085(92)80061-Q⟩. ⟨emse-00566875⟩