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In vitro and in silico study of cell growth in porous scaffold under dynamic flow

Abstract : The use of bioreactors for cultivating bone-forming cells on a three-dimensional porous scaffold material resolves mass transport limitations and provides physical stimuli, increasing the overall proliferation and differentiation of cells. Despite the recent and significant development of bioreactors for tissue engineering, the underlying mechanisms leading to improved bone substitutes remain mostly unknown. Previous studies have shown that numerical simulations can be a powerful tool to predict tissue development in complex environments. However, current models often present a poor representation of local physics and comparisons with experiments generally do not lead to a quantitative agreement. In order to experimentally reproduce the fluid flow through a porous scaffold, three-dimensional, micro-architectured micro-fluidic chambers have been designed. Osteoblast cells have been cultivated in micro-systems with and without flow, and cell proliferation dynamics have been monitored with image analyzing. Simultaneously, a numerical model has been developed in order to predict cell growth under fluid flow. Cell population dynamic is simulated using a three-dimensional cellular automaton, while the fluid flow is described using the Lattice-Boltzmann method (LBM). Experiments and numerical results show the influence of fluid induced shear stress on cell proliferation.
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Contributor : Nathalie Langlet <>
Submitted on : Monday, October 15, 2012 - 11:18:21 AM
Last modification on : Tuesday, April 20, 2021 - 4:54:04 PM


  • HAL Id : hal-00741428, version 1


M. Chabanon, H. Duval, O. Francais, Bruno Le Pioufle, E. Vennat, et al.. In vitro and in silico study of cell growth in porous scaffold under dynamic flow. 3rd TERMIS World Congress 2012 - Vienna, Austria, Sep 2012, Vienna, Austria. pp.340-341. ⟨hal-00741428⟩



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